Algebraically closedness on a theorem of Hartshorne.

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The following theorem is from Hartshorne's book on ample subvarieties:

Some definitions:

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The theorem:

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This is Corollary 1.2:

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This is prop 1.3: (The proof of this one is very long not sure if I can copy paste pages of proof here)

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Hartshorne always assumes he is working on algebraically closed fields and sometimes the assumption is not necessary. I was wondering whether this result is true if we drop the assumption that we are working on an algebraically closed field or not? I wasn't able to find where it is used exactly but it could be implicit somewhere. If true without the algebraically closed-ness I wonder whether there is a reference for it?