For what follows, my definition of a quasi-affine subset of $\mathbb{A}^n_F$ is one which can be written as $Z_1\setminus Z_2$, where $Z_1$ and $Z_2$ are closed subsets of $\mathbb{A}^n_F$. (I think this is also referred to being locally closed in some places?)
A few weeks ago I asked a question whether quasi-affine subsets of $\mathbb{A}^n_F$ ($F$ algebraically closed) are necessarily open or closed. This doesn't seem to hold for $n\geq 2$, so just as a follow up,
If $V=Z_1\setminus Z_2$ is a quasi-affine subset of $\mathbb{A}^1_F$, is $V$ necessarily open or closed in $\mathbb{A}^1_F$?
Hint: you can determine all closed subsets of $\mathbb{A}^1$ fairly easily.
How?
Full solution:
Conclusion (can't make the spoiler text work without this linebreak):