I am a beginner for set theory and I find something interesting:
Let $ U $ be a Grothendieck universe, then $ (U, \subseteq) $ is a partially ordered set. For every nonempty chain $ \mathcal{K} $ on it, $ \bigcup\mathcal{K} $ is an upper bound of $ \mathcal{ K } $. Thus by Hausdorff's maximal principle, which is equivalent to axiom of choice, the partially ordered set $ (U,\subseteq) $ has a maximal element $ M $. Put $ M^+=M\cup\{M\} $, since $ U $ is a Grothendieck universe, so $ M^+ $ is in $ U $. But by axiom of regularity, we have $ M \subsetneq M^+ $, and this contradicts with that $ M $ is a maximal element.
Does this means that the existence of Grothendieck universe contradicts with axiom of choice? Has this ever been considered before?
The problem with this proof is that you don't necessarily have that $\bigcup \mathcal{K}$ is in $U$ again. Universes aren't closed under all unions, just unions indexed by elements in $U$.
For example, if we consider $V_\omega$ (the collection of hereditarily finite sets) as a countable Grothendieck universe and let $0 = \emptyset$ and $n+1 = n \cup \{n\}$, then the collection $\{0, 1, 2, 3 \dots\}$ is a chain in $V_\omega$, but the union is an infinite set, hence not in $V_\omega$.