Fisher Information. Density function.

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I'm reading about Fisher's information. However, a question arises. What is the difference between the density function $$f(x,\theta)$$ and the same density function when composing with the random variable X?$$f(X,\theta)$$

$$I(\theta)=E_\theta\left[-\frac{\partial^2 }{\partial \theta^2}\ln f(\theta;X)\right].$$