Let $u$ be a function in $L^{2}(\mathbb{R}^{3})$,
Question1:
Is $\left \| u \right \|_{L^{2}} =0 $ implies that $F\left(u\right)=0$?
Question2:
Is $\left \| u \right \|_{L^{2}} > 0$ implies that there exists a positive constant $c> 0$ such that $F\left ( u \right )> c$?
Note that $F(u)$ is the Fourier transform of $u$.
The answer for question 1 is yes, because the Fourier transform $\mathcal{F}:L^2\to L^2$ is an isomorphism between Hilbert spaces. In particular for linearity it maps 0 to 0 (actually you only need $\mathcal{F}$ to be a linear mapping, which is easy to verify).
Remark that the $0$ of $L^2$ is a class of equivalence modulo a.e. equality, so you would have $\mathcal{F}(u)=0$ a.e.
The second part is false. To see it, take any $u\in L^2, u\ne0$ (and hence with strictly positive norm) and consider $\mathcal{F}(u)$. If there is a constant $C$ for which $\mathcal{F}(u)>C$, then $\mathcal{F}(-u)=-\mathcal{F}(u)<-C<0$ and $-u$ and $u$ have the same norm.