$P(\mathbb N)$ = power set of $\mathbb N$.
$A \subset P(\mathbb N)$ is a chain if $a,b \in A \implies$ either $a \subseteq b$ or $ b \subseteq a$
That is, we have something like this:
$$\cdots a \subseteq b \subseteq c \subseteq\cdots$$ where $a,b,c \in A$ are distinct.
We can show easy enough that there is an uncountable chain - this is done by noting $\mathbb N\sim\mathbb Q$ then using Dedekind cuts in $\mathbb Q$ to define $\mathbb R$ we see that a family of (nearly arbitrary) cuts satisfy the condition.
For instance the family $L_r=\{q \in \mathbb Q : q < r \}$ for $r > 0$ gives us the sets we need and obviously we can pick others.
I tried doing this for $ \mathbb R$ and don't seem to be getting anywhere.
To be more specific, does there exist a chain in $P(\mathbb R)$ with cardinality $2^ \mathbb R$? Given a set of cardinality $X$ (necessarily non-finite), is there a chain in $P(X)$ of the same cardinalty of $P(X)$?
Consider the tree $T=2^{\lt\omega_1}$, consisting of all countable ordinal length binary sequences. The number of such sequences is precisely the continuum, since there are $\omega_1$ many levels, each of size continuumn. The lexical order on this tree (or on any two ordinal-length binary sequences), compares them by the order of their least differing bit, placing the shorter one below when one is an initial segment of the other.
Notice that any path $p\in 2^{\omega_1}$ through the tree determines a cut in the lexical order, namely, the collection $X_p$ of $s\in T$ which preceed $p$ in the lexical order. Furthermore, if $p$ preceeds $q$ lexically, then $X_p\subset X_q$, and so we have a chain in $P(T)$ of size $2^{\omega_1}$.
Thus, without any additional assumption, this establishes chains in $P(\mathbb{R})$ of size at least $2^{\omega_1}$.
In particular, if the continuum hypothesis holds, then $|\mathbb{R}|=\omega_1$, and so this shows that there is a chain in $P(\mathbb{R})$ of size $2^{\mathbb{R}}$. So an affirmative answer to your question is a consequence of CH and thus consistent with the axioms of ZFC.
More generally, we obtain a positive answer under the much weaker assumption merely that $2^{\lt\frak{c}}=\frak{c}$, an assumption that follows from but is much weaker than CH. For example, this assumption is compatible with $2^\omega=\aleph_{15}$, if also $2^{\aleph_{14}}=\aleph_{15}$. In this case, the tree $T=2^{\lt\frak{c}}$ has size $\frak{c}$ and $2^{\frak{c}}$ many branches. And so the cuts determined by the lexical predecessors of paths through this tree gives rise to a chain of size $2^{\frak{c}}$ in $P(\frak{c})$, as desired.