Let $f \in H^{1} = H^{1}(I)$ with $|I| < \infty$. Then, $$ \|f\|_{H^{-1}} = \|f\|_{H^{1}} $$ where $H^{-1}(I) = (H_{0}^{1})^{\prime}$ and $\|f\|_{H^{1}} = \|f\|_{L^{2}(I)} + \|f^{\prime}\|_{L^{2}(I)}$.
I know that $L^{2}(I) \subset H^{-1}(I)$ with continuous injection, then there is $C_{1}>0$ such that $\|f\|_{H^{-1}} \leq C \|f\|_{L^{2}(I)} \leq C_{2}\|f\|_{H^{1}}$ for $C_{2} > 0$.
On the other hand, since $f \in H^{-1}$ then there is $f_{0} \in L^{2}(I)$ such $$ \|f\|_{H^{-1}} = \|f_{0}\|_{L^{2}(I)}. $$ But, how to show that there is $C_{3} > 0$ such that $\|f\|_{H^{-1}} \geq C_{3}\|f\|_{H^{1}}$?
Let $f\in H^1$, and note that $f\in H^{-1}$ can be represented by an operator $T_f:H^{1}_0\rightarrow \mathbb{R}$ such that $T_f(g)=\left<f,g \right>_{H^{-1}\times H_0^1}=\left< f,g \right>_{H_0^1}$.(For example, see Riesz representation theorem.) Then observe that \begin{align*} \left| \left< f,g \right>_{H^{-1}\times H^{-1}} \right|\leq \|f\|_{H_0^1}\|g\|_{H_0^1}. \end{align*}
It implies that $\|f\|_{H^{-1}}\leq \|f\|_{H_0^1}$.
Also, in the case when $g=f$, we have \begin{align*} |T_f(f)|=|\left<f,f \right>_{H^{-1}\times H_0^1}| = \|f\|_{H_0^1}^2 \end{align*} which implies that $\|f\|_{H_0^1}=\|f\|_{H^{-1}}$.
Here, I used $$\|f\|_{H_0^1}^2 = \int |\nabla f|^2$$ which is equivalent to with your norm by Poincare inequality.
Thus, in your representation, it would be $\|f\|_{H^{-1}}< \|f\|_{H^1}$ unless $f=0$ almost everywhere.