Is there a proof that a matrix is invertible iff its determinant is non-zero which doesn't presuppose the formula for the determinant?

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Proofs of the fact that a matrix is invertible iff its determinant is non-zero generally begin by saying "Define the determinant to be [very complicated formula]. We will now prove the result...". This is obviously unsatisfactory to many people.

Other proofs begin by listing axioms that the determinant should verify, and then prove that such a function exists (and is unique) and is given by the given formula. This isn't much better - why should we be interested in these axioms? Without knowing better, there isn't even any reason to suspect there exists any polynomial or simple function at all such that $f(A)=0$ iff $A$ is singular.

If you apply Gaussian elimination to a general 2x2 or 3x3 matrix, you get tantalizingly close, because the determinant formula arises naturally from the calculations, and it's clear that indeed, if it's zero, the matrix must be invertible. The trouble is that in order to get to that point, you need to start assuming that this, that and the other is non-zero so that you can divide by them, and your assumptions begin branching: if that's non-zero, then assume this is non-zero, otherwise... So it's difficult to see how this could be turned into a proper proof.

Can the proof outlined in the last paragraph be carried out rigorously? Or something like it?

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Yes, by preforming row operations the determinant is multiplied by a nonzero constant. Thus it suffices to prove this for reduced row echelon matrices. Such a matrix is either the identity or has a zero row.

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Let me work over the complex numbers. You can take the approach which I think is described in Axler: show that every square matrix over $\mathbb{C}$ can be upper triangularized (which can be done cleanly and conceptually: once you know that eigenvectors exist, just repeatedly find them and quotient by them), and define the determinant to be the product of the diagonal entries of an upper triangularization. Show that this doesn't depend on the choice of upper triangularization. Now it's very easy to check that an upper triangular matrix is invertible iff its diagonal entries are nonzero.

What this proof doesn't show is that the determinant is a polynomial in the entries, though.