I am following Herstein's Topics in Algebra and according to that book a module may be defined on a ring without 1 (https://i.stack.imgur.com/FMeIZ.png).
So if we consider a module $M$ over a ring $R$ without $1$, then will be the statement in the question true ? If yes , then how to prove it ?
You prove it exactly as in the case of modules over rings with unity.
In order to show the equivalence between injectivity of a map and triviality of the kernel you only use the fact that $$ f(x-y)=f(x)-f(y). $$ Mind that you still have $-y$ and $f(-y)=-f(y)$ even though you don't have $1$ (and so $-1$) because a module is nonetheless an additive group.