Lie algebra of nilpotent Lie group

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Let $G$ be a nilpotent closed subgroup of the group $UT(n,\mathbb{R})$ of upper triangular matrices with $1$-s on the diagonal.

I can construct a Lie algebra from $G$ in two ways:

  1. The usual Lie algebra of a matrix Lie group consisting of matrices $A\in M_n(\mathbb{R})$ for which $\exp(tA) \in G$.

  2. I can take the lower central series of $G$, take a direct sum of the factors and define Lie brackets in the natural (grading respecting) way.

Are the two Lie Algebras isomorphic? If so, canonically? (I'm new to this business)

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No. If the Lie algebra (i.e. the first construction) is denoted $\mathfrak{g}$, then the second one will be isomorphic to the associated Carnot-graded Lie algebra $\mathrm{Car}(\mathfrak{g})$.

Since there exist finite-dimensional nilpotent real Lie algebras (in dimension $\ge 5$) $\mathfrak{g}$ that are not isomorphic to their associated Carnot-graded Lie algebra, and since any finite-dimensional nilpotent real Lie algebra is isomorphic to a subalgebra of $\mathfrak{ut}(n,\mathbf{R})$ for some $n$, you get a negative answer to your question (at least for $n$ large enough, probably for all $n\ge 4$ actually).