Assume that $X\in \mathrm{PSD}(n)$ is a symmetric, positive semi-definite matrix with real entries and $C>0$ is a positive real number. If $X$ satisfies $$ \forall \alpha\in\mathbb{Z}^n\setminus\{0\},\qquad \alpha^T X \alpha \geq C, $$ then is it true that $X$ is positive definite? That is, if $X$ is lower bounded on integer vectors, is it true that it is lower bounded on whole $\mathbb{R}^n$? Can we give a concrete lower bound for $\lambda_{\min}(X)$ in terms of $C,n$ and $\lambda_{\max}(X)$?
The way I understand, the problem is closely related to approximations of real vectors with rational numbers: Simultaneous version of the Dirichlet's approximation theorem implies that if $v\in\mathbb{R}^n$ is a real vector and $N>0$ is a natural number, then there exists a rational vector $\beta\in\mathbb{Q}^n$ such that $$ \beta=\Big(\,\frac{p_1}{q},\, \frac{p_2}{q},\,\dots,\,\frac{p_n}{q}\,\Big)\; \text{ with }\;1\leq q\leq N\qquad\text{and}\qquad \Vert v-\beta\Vert <\frac{\sqrt{n}}{q N^{1/n}}. $$ Note that $\beta^T X \beta \geq \frac{C}{q^2}\geq \frac{C}{N^2}$. Since $\Vert v-\beta\Vert < \frac{\sqrt{n}}{q N^{1/n}}$ and $\beta^T X\beta\geq \frac{C}{N^2}$, I guess that choosing a large enough $N$ (relative to $\lambda_{\max}(X)$?) should guarantee that $v^T Xv> 0$.
Above, I am not using the sharpest bound so maybe Dirichlet's theorem might be an overkill.
Given $X \succeq 0$ and $C>0$,
$$\color{blue}{\forall \alpha\in\mathbb{Z}^n\setminus\{0\},\, \alpha^T ( X)\alpha \geq C \Rightarrow \, X \succ 0}.$$
Proof. If $X \succeq 0$, but $X \nsucc 0$, then there is a non-zero point $\beta\in\mathbb{R}^n\setminus\{0\}$ such that $\beta^T ( X)\beta=0.$ Thus, $\alpha^T ( X)\alpha$ is zero on the line $\alpha=t\beta, \, t\in\mathbb{R}$. Then, we have (see Lemma 1)
$$\sqrt{C}-0\le \sqrt{\alpha_\delta^T X\alpha_\delta} -\sqrt{(t_\delta\beta)^T X(t_\delta\beta)} \le \sqrt{\lambda_{\max}(X)} \|\alpha_\delta-t_\delta\beta \|_2 \le \sqrt{\lambda_{\max}(X)} \delta $$
where $\alpha_\delta \in\mathbb{Z}^n\setminus\{0\}$ and $t_\delta \in\mathbb{R}$ are chosen such that $\|t_\delta\beta-\alpha_\delta \|_2 \le \delta$, proven to exist in Lemma 2. If $\lambda_{\max}(X)=0$, the above gives a contradiction, and if $\lambda_{\max}(X)>0$, for $\delta < \sqrt{\frac{C}{\lambda_{\max}(X)}}$, we have a contradiction. Consequently the implication holds.
Lemma 1. $$\sqrt{\alpha^T X\alpha} -\sqrt{\beta^T ( X)\beta} \le \sqrt{(\alpha-\beta)^T X(\alpha-\beta)} \le \\ \sqrt{\lambda_{\max}(X)} \|\alpha-\beta \|_2 $$
Proof. The left inequality follows from that $\sqrt{\alpha^T X\alpha}$ is a semi-norm for $X \succeq 0$. The right inequality is obtained from the following inequality for a symmetric matrix $X$: $$\lambda_{\min}(X) \alpha^T \alpha \le \alpha^T X\alpha \le \lambda_{\max}(X) \alpha^T\alpha.$$
Lemma 2. Given $\beta \in\mathbb{R}^n\setminus\{0\}$, for any $\delta>0$, there is an integer vector $\alpha_\delta \in\mathbb{Z}^n\setminus\{0\}$ and $t_\delta \in \mathbb R$ such that $$\|\alpha_\delta-t_\delta\beta \|_2 \le \delta;$$ in other words, there are integer vectors that have arbitrarily small Euclidian distances to some points on the line $t\beta, t\in \mathbb R$.
Proof. It follows from the simultaneous version of the Dirichlet's approximation theorem, stated also in the OP, by setting $t_\delta=q$ and $\alpha_\delta=p$ where the rational vector $\frac{1}{q}p$ is obtained to approximate $\beta$ for $N$ satisfying $$\frac{\sqrt{n}}{ N^{1/n}} \le \delta, \frac{1}{ N^{1/n}N} < \|\beta\|_\infty=\max_{i\in[n]}|\beta_i|.$$ The latter is required to guarantee that $p\neq0$.