The Riemann-Liouville integral is defined by $$ I^\alpha f(x)=\frac{1}{\Gamma(\alpha)} \int_a^x f(t)(x-t)^{\alpha-1} d t $$ where $\Gamma$ is the gamma function and $a$ is an arbitrary but fixed base point. Take $a = 0$ and $\alpha = 1/2$. Therefore we look at: $$ I^{\frac{1}{2}} f(x) := \frac{1}{\Gamma(1/2)} \int_0^x \frac{f(t)}{\sqrt{x-t}} d t $$ Suppose $I^{\frac{1}{2}}f(x) = 0$ for all $x$. Can we then conclude $f=0$ a.e.?
My approach so far has been to take the Fourier transform and use the convolution theorem. I cannot conclude because I do not know if $f \in L^2(\mathbb{R})$. Otherwise, I could conclude just by using the fact that the Fourier transform is an isometry between $L^2$ spaces. See here for the same question on MathOverflow.
Assume $f \in L^{1}(\lambda)$, where $\lambda$ is the Lebesgue measure. First, use that (property of Riemann-Liouville integral)
$$I^{\alpha} (I^{\beta} f(x)) = I^{\alpha + \beta} f(x)$$
Therefore: $I^{1/2} (I^{1/2} f(x)) = I^1 f(x) = \int_{0}^{x} f(t) dt =0$. Then use (an adaptation of) Theorem 2.1 from these notes or this under the assumption that $f$ is integrable wrt to the Lebesgue measure. From this conclude that $f =0$ a.e.
I don't think it is possible to circumvent the assumption $f \in L^{1}(\lambda)$.
Edit: I found an even easier solution. The claim follows from a direct application of Titchmarsh convolution theorem, that you can find here or, for an even more useful extension of this result, see here.