How can I prove the Gamma function has no zeros in its holomorphy domain $\Bbb C\setminus\Bbb Z_{\le0}$ using only its integral definition $\Gamma(z)=\int_0^{+\infty}t^{z-1}e^{-t}\,dt$ valid when $\Re z>0$ and the functional equation $\Gamma(z+1)=z\Gamma(z)$?
From the integral definition we can find easily the holomorphic extension; thus it would be enough to prove that $\Gamma\neq0$ in $\{\Re z>0\}$, using thus the integral form. But I can't prove neither this.
Can someone help me?
EDIT: This question is not a duplicate because all the solution given use more "advanced" tools. Here I'm asking to prove that Gamma has no zeros using ONLY its integral representation
Assume that $\Gamma(\alpha) = 0$ for some $\Re(\alpha) > 0$. Then for any $s \geq 0$, the substitution $ t = (1+s)x$ gives
$$ 0 = \frac{\Gamma(\alpha)}{(1+s)^{\alpha}} = \int_{0}^{\infty} x^{\alpha-1} e^{-x} e^{-sx} \, \mathrm{d}x. \tag{1}$$
This is already enough to give a contradiction since the right-hand side is the Laplace transform of $x \mapsto x^{\alpha-1} e^{-x}$ and hence cannot be identically zero.
If we avoid the use of Laplace transform, still we can derive a contradiction. Let $0 < \sigma < \Re(\alpha)$. For this $\sigma$, we know that $\Gamma(\sigma) > 0$. Then multiply both sides of $\text{(1)}$ by $s^{\sigma-1}/\Gamma(\sigma)$ and integrate w.r.t. $s$ on $[0, \infty)$. By the Fubini's theorem, this yields \begin{align*} 0 & = \int_{0}^{\infty} x^{\alpha-1} e^{-x} \left( \frac{1}{\Gamma(\sigma)} \int_{0}^{\infty} s^{\sigma-1} e^{-sx} \, \mathrm{d}y \right) \, \mathrm{d}x \\ &= \int_{0}^{\infty} x^{\alpha-\sigma-1} e^{-x} \, \mathrm{d}x \\ &= \Gamma(\alpha-\sigma). \end{align*} This shows that $\Gamma(z) = 0$ along the line segment joining $i\Im(\alpha)$ and $\alpha$. Then the identity theorem tells us that $\Gamma(z)$ is identically zero for $\Re(z) > 0$, which is impossible.