For any given directed graph, we may consider the various closures of it with respect to reflexivity, symmetry, and transitivity, in any combination, like this:
For the particular graph shown above, this process results in eight distinct graphs, including the original graph. This graph is not the smallest instance with this feature, however, since if we delete the source point at right, we will still have eight distinct graphs, like this:
Question. What is the smallest directed graph such that these various closures are all distinct and distinct from the original?
The second example gets it down to five vertices and four edges.
The question arose in a reply of Bryan Bischof to my recent tweet https://twitter.com/JDHamkins/status/1318447368732397569. The first image is drawn from the chapter on Functions and Relations in my book, Proof and the Art of the Mathematics, available from MIT Press: https://mitpress.mit.edu/books/proof-and-art-mathematics.


The $4$-vertex digraph
is the smallest example possible.
To have the reflexive symmetric transitive closure be different from the symmetric transitive closure, we need an isolated vertex. (If a vertex $v$ has an edge to or from it, then in the symmetric transitive closure, we get the edge $v \to v$.) That isolated vertex will make all the reflexive closures different from the non-reflexive ones, but can't help us with anything else.
For the digraph
a ---> b ---> cwe can check that symmetric, transitive, and symmetric transitive closures are all different. If we want to beat this, we need the same thing to happen on a $2$-vertex digraph.If the $2$-vertex digraph has edges $a \to b$ and $b \to a$, then its symmetric closure will not change anything. However, if the $2$-vertex digraph does not have both of those edges, then its transitive closure will not change anything. So either way, we need $3$ vertices.