Assume $f\left(t,x\right)$ is $C^1\left(\left[0,T\right]\times\mathbb{R}, \mathbb{R}\right)$ and that the process $f\left(t, B_t\right)$ is a martingale w.r.t $\mathcal{F_t}=\sigma\left(B_s, s\leq\,t\right)$, with $B_t$ a standard Brownian motion.
Can we apply the Itô formula to get an expression for $d f\left(t,B_t\right)$ while we have only $C^1$ differentiability?
If yes, can we relax the conditions even further by assuming $f$ only $C^1$ differentiable w.r.t to $x$ since the derivatives $\frac{\partial}{\partial t}$ and $\frac{\partial^2}{\partial x^2}$ should in principle be unnecessary since $f\left(t,B_t\right)$ is a martingale?
Of course it ought to be the case that $f(t,B_t) =\int_0^t f'_2(s,B_s)\,dB_s$, where $f'_2$ is the partial derivative of $f$ with respect to its second variable. And this is indeed true. First note that by localization one can reduce to the case in which $f$ and both of its first-order partials are bounded. Things being so, the continuity of $f$ and those partials allow one to use the argument in section IV.41 of volume 2 of Diffusions, Markov Processes, and Martingales by Rogers and Williams demonstrating Clark's Formula for the integrand in the stochastic integral representation of a Brownian functional. That argument shows that $$ f(T,B_T) =f(0,B_0)+\int_0^Tf'_2(s,B_s)\,dB_s $$ almost surely. Now take conditional expectations with respect to $\mathcal F_t$ in this identity to obtain $$ f(t,B_t) =\Bbb E[f(T,B_T)|\mathcal F_t] = f(0,B_0)+\int_0^t f'_2(s,B_s)\,dB_s, $$ a.s. for each fixed $t\in[0,T]$. As the extreme terms in this last display are a.s. continuous functions of $t$, it follows that $$ f(t,B_t) = f(0,B_0)+\int_0^t f'_2(s,B_s)\,dB_s,\qquad\forall t\in[0,T], $$ almost surely.