problem statement: Cutomers arrive at a store according to a Poisson distribution with $\lambda = 1$. Suppose we observe customer arrivals from time t = 0 to t = T and find only 1 customer arrived during this period. What is the probability that this single customer arrived between t = 0 and t = T/4?
The possible answers are:
Since it is just a single customer that arrives in that time period, and assuming this person arrives uniformly, isn't the answer simply 1/4?
How does $\lambda$ come in to play here? And what does $\lambda = 1$ mean? 1 person per minute, 1 per per hour, 1 person per T?

Yes, however, the property that determines this is the independence of arrivals, not that they arrive uniformly; they don’t or at least the don’t necessarily.
It doesn’t.
1 person per unit period in whatever period λ is expressed in. The question doesn’t tell us.