Define $$\langle X,Y \rangle := \operatorname{tr}XY^t,$$ where $X,Y$ are square matrices with real entries and $t$ denotes transpose.
I have some troubles in proving that $$ \langle [X,Y],Z \rangle = - \langle Y,[X,Z] \rangle,$$ where square brackets denote commutator.
Let me update my questin to part ii. You have proven that my commutation relation without a transpose is wrong, while it is correct if we put a $t.$
Then I'd say I'm in trouble, because the next step would be to define $$\operatorname{ad}_XY:=[X,Y]$$ and claim that by the above (false) property we have that $\operatorname{ad}$ is antisymmetric, i.e. $$\langle \operatorname{ad}_XY,Z\rangle =- \langle Y,\operatorname{ad}_XZ\rangle:$$ Do you know of a way to recover such a nice property or something similar?
The key properties to use are
$$\langle A,B\rangle=\langle B, A\rangle,$$
i.e. with $tr((AB)^t)=tr(AB),$ and
$$tr(ABC^t)=tr(BC^tA),$$
for all $A,B\in M_n(\mathbb R)$.