Let's assume we have a partial or total order relation $R$ defined on a set $S$.
If $R$ was not strict (i.e. it denoted $\leq$ instead of $<$), an equality relation $E$ could be defined as such:
$$ xEy:=xRy\land yRx $$
In other words:
$$ x=y:=x\leq y\land y\leq x $$
For all $x,y\in S$.
But if $R$ was strict (i.e. it denoted $<$ instead of $\leq$), I can't think of a way that $E$ could be defined using $R$. Is it possible to do so?
For a total order, yes. $xEy \iff (\lnot (x R y) \land \lnot (y R x))$. I don't see any way to use a strict partial order to define equality.