The constructible powerset is defined in Wikipedia as:
$$\operatorname{Def}(X) := \Bigl\{ \{ y \in X \mid (X,\in) \models \Phi(y,z_1,\ldots,z_n) \} \Big| \Phi \text{ is a wff and } z_{1},\ldots,z_{n} \in X \Bigr\}.$$
My question is whether this operator can be defined without the "models" notion being used here, only basic set operations. For comparison, consider the finite axiomatization of NBG. In the usual axiomatization we have the "single" axioms: extensionality, pairing, union, powerset, infinity, and limitation of size; plus one axiom schema for comprehension. It was later shown that this axiom schema can be replaced by some eight or so "Godel operators", each of which performs some simple set theoretic operation like $\{(b,(a,c))\mid(a,(b,c))\in R\}$, and together these operations can build anything that the original comprehension schema could produce.
I think that, in his book on the consistency of the continuum hypothesis (not the brief paper in the Proceedings of the National Academy but the book), Gödel uses what are now called the Gödel operations to define the constructible universe. I think Shoenfield's book "Mathematical Logic" uses the same approach. So the answer to the title of your question is yes. The body of your question asks for something more difficult, not just getting $L$ but getting the individual stages of the constructible hierarchy. That can undoubtedly be done by similar methods, but I don't recall ever seeing it worked out. It would be tedious work, and I suspect nobody cared about it enough to do the work.