For each $n\in\mathbb N$, let $A_n$ be the $n\times n$ matrix with entries $$ A_n(k,j)=\begin{cases} 1,&\ k+j=n+1\\ 1,&\ k+j=n+2,\\ 0,&\ \text{ otherwise} \end{cases} $$ So, for instance, $$ A_4=\begin{bmatrix} 0&0&0&1\\ 0&0&1&1\\ 0&1&1&0\\ 1&1&0&0\end{bmatrix}. $$ I'm looking for a proof of the following:
If $n=2m$, then $A_n$ has $m$ positive and $m$ negative eigenvalues.
if $n=2m+1$, then $A_n$ has $m+1$ positive and $m$ negative eigenvalues.
Finding the eigenvalues explicitly is definitely not an option, judging by the formulas already in the case $n=3$.
If $p_n$ denotes the characteristic polynomial of $A_n$, then we have the recursion $$ p_{n+1}(t)=-t\,p_n(t)-p_{n-1}(t), $$ but I don't know if one can obtain meaningful information from this.
With a bit of help from a friend, I found this way to answer the question.
Consider first the case $n$ even, say $n=2m$. Then $A_n$ has an $n\times n$ block of zeroes in the upper left corner. Cauchy's Interlacing then gives (note that $A_n$ is invertible for all $n$) $$ \lambda_k(A)<0<\lambda_{2m-m+k}=\lambda_{m+k}. $$ So the first $m$ eigenvalues of $A_{2m}$ are positive, and the other $m$ are negative.
When $n$ is odd, $n=2m+1$, we have $$ A_{2m+1}=\begin{bmatrix} 0_{m\times m} & E & Y\\ E^*&\begin{bmatrix} 1&1\\1&0\end{bmatrix}&0_{2\times (m-1)}\\ X&0_{(m-1)\times 2}& 0_{(m-1)\times (m-1)} \end{bmatrix}, $$ where $E$ is the $m\times2$ matrix with $1$ in the $m,2$ entry and zeroes elsewhere; $Y$ is the $m\times(m-1)$ matrix (using matrix unit notation) $$ Y=E_{1,m-1}+E_{m,1}+\sum_{j=2}^{m-1}E_{j,m-j+1}+E_{j,m-j}, $$ and $$ X=\sum_{j=1}^{m-1}E_{j,m-j+1}+E_{j,m-j} $$
For an example, consider $m=3$: $$ \left[ \begin{array}{ccc|cc|cc} 0&0&0&0&0&0&1\\ 0&0&0&0&0&1&1\\ 0&0&0&0&1&1&0\\ \hline0&0&0&1&1&0&0\\ 0&0&1&1&0&0&0\\ \hline0&1&1&0&0&0&0\\ 1&1&0&0&0&0&0 \end{array} \right]. $$ Now define $$ V=\begin{bmatrix} I_{m }&0_{m\times 2}&0_{m\times(m-1)}\\ 0_{2\times m}&\begin{bmatrix} 1&0\\-1&1\end{bmatrix}& 0_{2\times(m-1)}\\ 0_{(m-1)\times m}&0_{(m-1)\times 2}& I_{m-1} \end{bmatrix}. $$ One then checks $$\tag1 VA_{2m+1}V^*=\begin{bmatrix} 0_{m\times m}&E&Y\\ E^*&\begin{bmatrix}1&0\\0&-1\end{bmatrix}& 0_{2\times(m-1)}\\ X&0_{(m-1)\times2}& 0_{(m-1)\times(m-1)} \end{bmatrix} $$ By Sylvester's Law of Inertia, the matrix in $(1)$ has the same number of positive and negative eigenvalues as $A_{2m+1}$. If we now look at row and column $m+1$, we see that both are zero with the exception of the $1$ at the $m+1,m+1$ entry. So, by conjugating with the right permutation (the one that flips rows $1$ and $m+1$), we obtain $$ \left[\begin{array}{c|ccc} 1&0_{m\times m}&0_{1\times m}&0_{1\times(m-1)}\\ \hline 0_{m\times1}&0_{m\times m}&e_m&Y\\ 0_{1\times m}&e_m^*&-1&0_{2\times(m-1)}\\ 0_{(m-1)\times1}&X&0_{(m-1)\times1}&0_{(m-1)\times(m-1)}. \end{array}\right] $$ This matrix has $1$ has an eigenvalue, and the remaining eigenvalues are those of the $2m\times2m$ block down right; this block is selfadjoint, and has an $m\times m$ block of zeroes, so reasoning as in the even case we conclude that it has $m$ positive and $m$ negative eigenvalues. In conclusion, $A_{2m+1}$ has $m+1$ positive eigenvalues and $m$ negative eigenvalues.