Let $C$ and $D$ be categories, let $F$ and $G$ be functors from $C$ to $D$, and let $\gamma$ and $\delta$ be natural transformations from $F$ to $G$. Then my question is, if $\gamma_a=\delta_a$ for some $a\in C$, then is it necessarily true that $\gamma=\delta$? In other words, is a natural transformation uniquely determined by a single morphism?
If it’s not true in general, is it at least true if all the morphisms in $D$ are isomorphisms? I think it might be implied by the naturality condition.
No. Let $C$ be a discrete category. Then a natural transformation/isomorphism is just a $C$-indexed family of morphisms/isomorphisms of $D$. The naturality condition becomes vacuous.