I have noticed that the following simple sum of Laguerre polynomials (weighted with alternating signs) seems to be positive for any $N$ when $x>0$:
$$\sum_{k=0}^{N}\;(-1)^{k}\;L_{k}(x)$$
More precisely, when $N$ is even, the sum is positive $\forall\;x$ and when $N$ is uneven, the sum has only one zero in $x=0$.
I have checked this numerically up to $N=10{}^{2}$.
I cannot find any reference to this sum in the literature. Are my observations correct? If this is a known result, could someone point me in the right direction to find a reference or some documentation for this?

Maybe some help on the way but not a full answer.
According to Wikipedia, Laguerre polynomials build a Sheffer sequence
$$\frac{\partial }{\partial x} L_n = \left(\frac{\partial }{\partial x}-1\right)L_{n-1}$$
Which can be rewritten as :
$$L_{n-1} = \frac{\partial }{\partial x}(L_{n-1}-L_n)$$
So by the fundamental theorem of calculus we can say
$$\int_0^xL_{n-1}(t)dt = L_{n-1}(x)-L_n(x)$$
So your sum is a pairwise sum of such integrals.
Remains to be proven that sum of such integrals for even $n-1$ are positive.