We have a canonical homomorphism from a ring $R$ to its field of fractions $Q(R)$ given by $f : r\mapsto r/1$.
Is this homomorphism unique?
So letting $g: R\rightarrow Q(R)$ be a homomorphism, by the universal property of the field of fractions, there exists a unique homomorphism $h:Q(R)\rightarrow Q(R)$ such that $g = h\circ f$. By this universal property it follows that it should be enough to prove that $h$ is the identity map, but I am not sure if this has to be the case.
Added part: As follows from the answers/discussion below, in general the above is not true. Now I wonder, is it true when $R$ is a discrete valuation ring?
There can be more than one ring homomorphism $R\to Q(R)$. Let's look at an example to see why:
Let $R$ be the polynomial ring $\Bbb{C}[t]$. So $Q(R)=\Bbb{C}(t)$. If we pick any element $r\in\Bbb{C}(t)$, there is a unique ring homomorphism $\phi_r:\Bbb{C}[t]\to\Bbb{C}(t)$ with $\phi_r(t)=r$ and $\phi_r(z)=z$ for all $z\in\Bbb{C}$. Note: $\phi_r$ is the map which sends $p(t)\mapsto p(r)$ for each polynomial $p(t)\in\Bbb{C}[t]$. So if $R=\Bbb{C}[t]$, then there are lots of ring homomorphisms $R\to Q(R)$.