If we define the real numbers abstractly as a complete ordered field (like described in the Wikipedia page), how can we prove that they are uncountable? In other words, using just the axioms of a complete ordered field, how can we show that any model satisfying those axioms is not isomorphic to N?
The usual proof I've seen of uncountability of $\mathbb R$ is through a diagonalization argument, but that involves a specific representation of $\mathbb R$ - namely infinite decimal sequences. How can we do it in the abstract, without that (or any other) specific representation?
There are many proofs of the uncountablity of the reals. Firstly though, note that the Cantor proof you mention only uses the axioms of complete ordered field, since the fact that decimal expansions exist is a consequence of those axioms.
You are perhaps looking for a proof that is more directly related to analytical properties of the reals, rather than any typographic representation of the reals. Well, Baire's theorem states that a non-empty complete metric space is not of first category. If the reals were countable, then they would be of first category. As some real exists, the reals are not countable.