The dynamical system ${\bf \dot x} = {\bf f}({\bf x})$ is called a gradient system if there exists a function $V({\bf x})$ such that $$ {\bf f}({\bf x}) = - \nabla V({\bf x}) $$ Show that if ${\bf \dot x} = {\bf f}({\bf x})$ is a gradient system, then $$ \frac{\partial f_i}{\partial x_j} - \frac{\partial f_j}{\partial x_i} = 0 $$ for $1 \leq i, j \leq d$
So let ${\bf f}({\bf x}) = (f_1({\bf x}), f_2({\bf x}), \dots , f_n({\bf x}))$ and from the definition of a gradient system, we have $$ (f_1({\bf x}), f_2({\bf x}), \dots , f_n({\bf x})) = - \left ( \frac{\partial V({\bf x})}{\partial x_1}, \frac{\partial V({\bf x})}{\partial x_2}, \dots, \frac{\partial V({\bf x})}{\partial x_n} \right ) $$ but then I don't see how to follow this to produce the required equation.
Hint
$V$ has two continuous derivatives, your equation is Schwarz' theorem in disguise. $$\frac{\partial^2}{\partial x_i\partial x_j} V = \frac{\partial^2}{\partial x_j \partial x_i} V$$ now write this in terms of $f$.