I know that the two norm of a matrix is the largest singular value.
I am presented with an operator $U$ where $U: \mathbb{R}^n \to \mathbb{R}^n$
$U = A\exp(x) = \text{diag}\{a_1, a_2, \ldots, a_n\}\begin{bmatrix} \exp(x_1) \\ \exp(x_2) \\ \vdots \\ \exp(x_n) \end{bmatrix}$ where $a_i > 0$
How do I evaluate $\|U\|_2$ in this case?
I'm afraid your operator does not have a finite norm. Consider, for example, the case $x_1=x_2=\ldots=x_n=c_1$. Then $\left\|x\right\|_2=c_1\sqrt{n}$. However, by your definition of $U$, we have $$\left\|Ux\right\|_2 = \sqrt{\sum_k a_k^2 {\rm e}^{2c_1}} = C_2 \cdot {\rm e}^{c_1},$$ where $C_2 = \sqrt{\sum_k a_k^2}>0$. Therefore, $$\frac{\left\|Ux\right\|_2}{\left\|x\right\|_2} = \frac{C_2}{c_1} {\rm e}^{c_1},$$ which grows unbounded for $c_1\rightarrow \infty$. Therefore, the supremum over $x$ does not exist.