Let $B=A[x]$ be an integral extension of a Dedekind ring $A$ where $x$ has minimal (monic) polynomial $f(x)$. Then the module of Kahler differentials $\Omega_A^1 (B)$ is generated by $dx$. Why is its annihilator $f'(x)$?
$\Omega_A^1 (B)=I/I^2$, where $I:=\{b\otimes_A 1 -1\otimes_A b| b\in B\}$. So the annihilator of $dx = x\otimes 1 - 1\otimes x$ should be $\{h\in B| hx\otimes 1 -h\otimes x \in I^2\}$. How do I proceed from here?
Let us write $B = A[t]/(f(t))$ where $f(t)$ is some monic polynomial with coefficients in $A$. Then (you should prove!) that the Kahler differentials are precisely $\Omega_{B/A} = B[dt]/ (f'(t)dt)$. It is now clear the annihilator of this $B$-module is precisely $(f'(t))$.