The following expression yields an integer answer (very surprisingly it seems).
$${\left (515391\frac{33}{40} \right)}^4 - {\left (384140\frac{39}{40} \right)}^4 = 48783404650404592720562$$
I have tried many other pairs of 6-digit integers , but none of them result in an integer answer. For example: $${\left (515390\frac{33}{40} \right)}^4 - {\left (384141\frac{39}{40} \right)}^4 = 48782630297643606282783.184$$
$${\left (515389\frac{33}{40} \right)}^4 - {\left (384142\frac{39}{40} \right)}^4 = 48781855946299371591451.728$$
$${\left (515392\frac{33}{40} \right)}^4 - {\left (384139\frac{39}{40} \right)}^4 = 48784179004582352493575.376$$
I have tried over a hundred pairs of 6-digit integers , but none of them result in an integer answer. It seems that a special property or characteristic of the pair of integers 515391 and 384140 , makes the above expression an integer. But what special property or characteristic ? Can anyone see why the above expression is an integer ?
Let $x=40(515391\frac{33}{40})$ and $y=40(384140\frac{39}{40})$. Your expression will be an integer if and only if $x^4-y^4$ is a multiple of $40^4=2^{12}\cdot 5^4$.
The fact that it is a multiple of $40^4$ can be seen by observing the following three things:
Why is this enough? It boils down to the factorization $x^4-y^4=(x-y)(x+y)(x^2+y^2)$:
So $x^4-y^4$ is a multiple of both $2^{12}$ and $5^4$, which is what we wanted.
Note that these three conditions are general enough to let you find lots of other pairs $(x,y)$ with $x^4-y^4$ a multiple of $40^4$ (though not all of them):
For example, we could take $N=2^{10} \cdot 123456789 = 126419751936$. Then $N \equiv 61 \pmod{5^4}$, and so we have to solve the equation $61 \equiv 31q \pmod{5^4}$. This equation has solution $q=506$, so we can take any odd $x \equiv 7 \cdot 506 \equiv 417 \pmod{5^4}$. Let's take $x=417 + 5^4 \cdot 98765432 = 61728395417$. Finally, $y=N-x=64691356519$. You can check that $$ \left(\frac{x}{40}\right)^4-\left(\frac{y}{40}\right)^4=\left(1543209885\frac{17}{40}\right)^4-\left(1617283912\frac{39}{40}\right)^4 $$ is an integer.
Based on your comment, you are specifically interested in $x,y$ which are congruent to 33 and 39 modulo 40. You can do that in the same way, but it takes a little extra work. Since $N=x+y$, we need to take $N \equiv 33+39 \equiv 32 \pmod{40}$ as well as being a multiple of $2^{10}$. It's not hard to check that this happens when $N=2^{10} \cdot p$ where $p \equiv 3 \pmod{5}$. For example, we could take $N=2^{10} \cdot 1000003=1024003072$.
You can check that, if we take $q=462$, then $31q \equiv N \pmod{5^4}$. So we want to choose $x$ with $x \equiv 7 \cdot 462 \equiv 109 \pmod{5^4}$ and either $x \equiv 33$ or $x \equiv 39 \pmod{40}$. The first of these is impossible (because it implies incompatible things about $x$'s mod 5 congruence class) but the second is possible: it happens whenever $x \equiv 1359 \pmod{5000}$. For example, we could take $x=400001359$, and then $y=1024003072 - x = 624001713$. Again, you can check that $$ \left(\frac{x}{40}\right)^4 - \left(\frac{y}{40}\right)^4 = \left(10000033 \frac{39}{40}\right)^4- \left(15600042\frac{33}{40}\right)^4\\ = -49224604028046128106617226275 $$ is an integer, which after swapping the order of the terms gives you what you want.