This is an exam question, testing if water is bad - that is if a sample has more than 2000 E.coli in 100ml. We have taken $n$ samples denoted $X_i$, and model the samples as a Poisson distribution with parameter $\theta$, and as mutually independent.
1 Determine the maximum likelihood estimator for $\theta$, denote it $\hat\theta_n$ from now on.
Under the Poisson distribution: $$ P(X=k)=\frac{\theta^k}{k!}e^{-\theta}\text{ so the likelihood is } L(\theta)=\frac{\theta^{\sum x_i}\, e^{-n\theta}}{x_1!\ldots x_n!}$$
$$ \ln (L(\theta)=\ln \left(\frac{\theta^{\sum X_i}\, e^{-n\theta}}{x_1!\ldots x_n!}\right)= \sum_{i=1}^n x_i\ln(\theta)-n\theta+\text{ term with no theta} $$ Now differentiating w.r.t. $\theta$: $$ \frac{\partial}{\partial\theta}\ln(\theta)=\frac{1}{\theta}\sum x_i-n\text{ and a second time }\frac{\partial^2}{\partial\theta^2}\ln(\theta) = \frac{-1}{\theta^2}<0$$ So the maximum likelihood estimator $\hat\theta_n=n^{-1}\sum{x_i}$
2 Calculate the expected value and variance: I got $\theta$ and $\theta/n$ respectively - which I think are right.
3 Show for $\epsilon>0$ $$ P(|\hat\theta_n-\theta|>\epsilon\sqrt{\theta})\leq\frac{1}{n\epsilon^2}$$ This follows from Chebyshev's inequality with the constant as $\epsilon\sqrt{\theta}$.
Now the bit I can't match up with my notes and complete: construct a confidence interval of level $\alpha$. We have covered Bienayme-Tchebychev and Hoeffding for confidence intervals of proportions, but I don't think that is right here. Can I use an interval of the form: $$\left[\hat\theta_n\pm\frac{1}{\sqrt{n}}\sqrt{\hat\theta_n (1-\hat\theta_n)}\,\phi^{-1}(1-\frac{\alpha}{2}) \right]$$ where $\phi$ is the inverse cdf of the standard normal?
The final part is to construct a test of level $\alpha$ for the null hypothesis the water is bad with the data $n=20$, $\sum_{i=1}^{20} X_i=38600$, $\alpha=0.05$ What decision is taken?
You are right, for this, you cannot use a CI based on proportions. Actually, the method of constructing CI based on proportions is a consequence of the Central Limit Theorem for Binomial Parameter Estimation. It isn't something arbitrary.
We use a result from the Central Limit Theorem.
This is the Delta Method.
What we will do is we will try to find a confidence interval for $g(\mu)$ rather than $\mu$ and hence get a confidence interval for $\mu$. To do that, our variance term for the CI for $g(\mu)$ must be a constant, say $1$. So you choose $g$ such that $(g'(\mu))^2Var(X_i)=1$, your $\mu$ being the argument for $g$.
In our case, $\mu=\theta$ and $Var(X_i)=\theta$ so we want to find $g$ so that $g'(\theta)=\dfrac{1}{\sqrt{\theta}}$, implying $g(\theta)=2\sqrt{\theta}$ as one of the solutions (no need to worry about the constant term as it is irrelevent; your job is to find $g$ which satisfies this, not its properties).
Thus, frame a CI for $g(\theta)=2\sqrt{\theta}$ using the CLT, which you should be able to do. You want to look at:
$$P[-c\leq \sqrt{n}(2\sqrt{Y_n}-2\sqrt{\theta})\leq c]=1-\alpha$$
You get your $c$ based on $\alpha$ and hence you get a confidence interval for $\theta$.
Please take some time and try to use this method to find a suitable level $\alpha$ test. Try for 1 day, after that if you still cannot do it, please comment below the answer. All the technicalities have been covered, now you should be able to peform the main inference based on your CI.