We are given a $\left((k+1)n - 1\right)\times \left((k+1)n-1\right)$ square grid and tiles of size $1\times n$. We can place the tiles anywhere on the board, provided that they never cover the same area more than once. The tile orientation (horizontal or vertical) does not matter.
The question asked is can we put some number of these tiles on the board so that there exists a $(kn+1)\times(kn+1)$ region which is fully covered by the tiles?
After a few attempts I suppose the answer is negative. I have tried various tricks with coloring the grid, but with no success. I think it's crucial that we can never fit $k + 1$ tiles oriented horizontally in the same row (an analogous observation is true for columns), so maybe there is a clever way of coloring based on that.
The question originally asked for the case $k=3, n=5$ under the assumption the $(kn+1)\times(kn+1)$ region must not share any border with the whole grid. However, as was suggested in the comments, this assumption is not necessary.
As hinted, here's a much simpler solution to the much simpler case, which allows for a much easier generalization. Through various tweaks of the setup, this resulted in a very different (to me) solution, which is why I posted it separately. I can delete the other solution if that is desired.
Consider the following coloring of the $ 5 \times 5 $ grid.
$$\begin{array}{ccccc} a &b &-a-b &a &b \\ c &d &-c-d &c &d \\ -a-c &-b-d &a+b+c+d& -a-c& -b-d \\ a &b &-a-b &a &b \\ c &d &-c-d &c &d \\ \end{array}$$
Observe that
Now, suppose that there are $1\times 3$ tiles that can cover a $ 4 \times 4$ area. Observe that
Now, for the generalization
Consider the following coloring:
Observe that
Now, suppose that there are tiles that can cover a $ (k-1) n + 1 \times (k-1)n + 1 $ area, which we'd refer to as $S$. Observe that
Note: With this coloring, we can even strengthen the $5 \times 5$ grid case, to show that we cannot even cover squares that include
$$\begin{array}{ccccc} x & & &x \\ &x &x & \\ & x &x & \\ x & & &x \\ \end{array}$$