How do I know that the eigenvalues of a Sturm-Liouville problem are simple or multiple?

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In a Sturm Liouville problem, how can we predict that it only has simple eigenvalues or it contains multiple eigenvalues? Do the boundary conditions such as 1) zero Dirichlet 2) periodic 3) semi-periodic matter? Do regularity/singularity matter?

Also, is there a general approach that able us to approximate the eigenvalues one by one in ascending order?