How to test significant differences?

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I have a model that generates results based on simulation with different settings. Now I want to know if it does really matter what settings I use. I have a single column of results, one result for each different settings. How can I test if there is a significant difference between the results?

For example: column 1

I looked at t-test, but I need a H_0, for that, and that is not applicable in my case.