Let $A$ be a square matrix each of whose columns has sum $1$. Let $B$ be the matrix obtained by replacing the lowest row in $A-I$ with a row of ones.
Can anybody show (or find a counterexample) that $-\det(B)$ is exactly the degree $1$ coefficient in the characteristic polynomial $\det(A-I-tI)$ ?
With a computer I have checked this for $n=2,3,4,5$.
Even though the hypothesis does not ask that the entries of $A$ be non-negative, the problem has a stochastic flavour, so I added a [stochastic-matrices] tag.
Let $P$ be the matrix obtained by filling the last row of $I$ by ones. Then $$ P(A-I)P^{-1}=\pmatrix{X&y\\ 0&0} \ \text{ and }\ P(A-I+e_ne^T)P^{-1}=\pmatrix{X&y\\ 0&1} $$ for some $(n-1)$-rowed square matrix $X$ and some vector $y$. Note that if you add the first $n-1$ rows of $A-I+e_ne_n^T$ to the last row, you get $B$. Therefore $$ \det(B)=\det(A-I+e_ne^T)=\det(X). $$ Hence the coefficient of $t$ in $\det(A-I-tI)=\det\left(P(A-I)P^{-1}-tI\right)=-t\det(X-tI)$ is $-\det(X)=-\det(B)$.