The localization $K[X]_{X}$ is a ring extension of $K[X].$ I want to show that $K[X]_X$ is not integral over $K[X]$ using lying above.
I tried to find a maximal ideal in $K[X]_X$ whose contraction in $K[X]$ is not maximal ideal, or maybe we can produce two prime ideals above one containing another whose contraction is the same prime ideal. I need some help to construct such prime ideals. Thanks.
Your second approach cannot work because this does not imply that the lying over property fails. For example, the extension $\Bbb{Z}\subset\Bbb{Z}[i]$ is integral and hence has the lying over property. But the two prime ideals $(2+i),(2-i)\subset\Bbb{Z}[i]$ satisfy $$(2+i)\cap\Bbb{Z}=(2-i)\cap\Bbb{Z}=5\Bbb{Z},$$ so their contraction is the same prime ideal.
Instead, consider the prime ideal $(X)\subset K[X]$. If there is a prime ideal $\mathfrak{q}\subset K[X]_X$ such that $\mathfrak{q}\cap K[X]=(X)$ then in particular $X\in\mathfrak{q}$. But $X$ is a unit in $K[X]_X$ and so $\mathfrak{q}=K[X]_X$, a contradiction. So there is no prime ideal lying over $(X)$.
For some more perspective; in general when localizing a (commutative unital) ring $R$ with respect to a multiplicative subset $S$, the set of prime ideals of $R_S$ corresponds bijectively to the set of prime ideals of $R$ that are disjoint from $S$. The bijection is given by taking contractions/extensions w.r.t. the localization map $R\ \longrightarrow\ R_S$.
In this particular case, the set of prime ideals of $K[X]_X$ corresponds bijectively to the set of prime ideals of $K[X]$ that do not contain any power of $X$. These are all prime ideals except $(X)$, and every prime ideal of $K[X]$ except $(X)$ is the contraction of a prime ideal of $K[X]_X$.
[Original answer, where I mistook $K[X]_X$ for $K[X]_{(X)}$.]
As you say, the ring $K[X]_X$ is local, so there is only one maximal ideal, which is the ideal generated by $X$. Its contraction in $K[X]$ is the ideal generated by $X$ there, which is also maximal, so this approach won't work.
The only other prime ideal of $K[X]_X$ is the zero ideal, which contracts to the zero ideal in $K[X]$, which is also prime. So this approach won't work either.
However, this does show that the extension does not have the lying over property; the ring $K[X]_X$ has only two prime ideals whereas $K[X]$ has infinitely many. So there must be some prime ideal of $K[X]$ that is not the contraction of a prime ideal of $K[X]_X$.