When do we have the formula $f(t)=e^{\lambda t}f(0)+\int_0^te^{\lambda (t-s)}g(s)ds$?

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Let $g:\mathbb{R}\to \mathbb{R}$ be a continuous function. Consider the following integral equation $$f(t)=f(0)+\int_0^t\lambda f(s)ds+\int_0^tg(s)ds. \tag{1}$$ Since $g$ is continuous, Thus the integral equation $(1)$ has a solution $f$ which is derivable everywhere and is given by: $$f(t)=e^{\lambda t}f(0)+\int_0^te^{\lambda (t-s)}g(s)ds,\tag{2}$$ because $(2)$ implies that $f$ satisfies the following differential equation $$f'=\lambda f+g,$$ and then satisfies $(1)$. (To be correct, we have a lot of solutions, since changing $f(0)$ gives other solutions)

Now if we assume that $g$ is only locally integrable. Do we still have a solution of the integral equation $(1)$ ? and can it be given also by the formula $$f(t)=e^{\lambda t}f(0)+\int_0^te^{\lambda (t-s)}g(s)ds.$$

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Suppose you have a locally integrable $g$ and a function $f$ such that \begin{align} f(t) & = e^{\lambda t}f(0)+\int_{0}^{t}e^{\lambda(t-s)}g(s)ds \\ e^{-\lambda t}f(t) & = f(0)+\int_{0}^{t}e^{-\lambda s}g(s)dt \end{align} The function $e^{-\lambda t}f(t)$ is locally absolutely continuous, which also means that $f$ must be locally absolutely continuous, and the following holds a.e.: $$ e^{-\lambda t}f'(t) -\lambda e^{-\lambda t}f(t) = e^{-\lambda t}g(t) \\ f'(t) -\lambda f = g \\ f'(t) = \lambda f + g. $$ Because $f$ is absolutely continuous, integration of $f'$ gives back $f$: $$ f(t)-f(0) = \int_{0}^{t} f'(s)ds = \lambda \int_{0}^{t}f(s)ds+\int_{0}^{t}g(s)ds \\ f(t) = f(0)+\lambda \int_{0}^{t}f(s)ds+\int_{0}^{t}g(s)ds. $$

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If $g$ is locally integrable, then the function $f(t):=e^{\lambda t}f(0)+\int_0^te^{\lambda(t-s)}g(s)\,ds$ is well-defined and continuous, and it's not difficult to show that this $f$ is a solution of your integral equation $f(t) = f(0)+\int_0^t\lambda f(s)\,ds+\int_0^t g(s)\,ds$ (using Fubini's theorem).

If $\tilde f$ is a second (locally integrable) solution of the integral equation, with the same initial value, then the difference $h(t):=f(t)-\tilde f(t)$ satisfies $h(t) =\lambda\int_0^t h(s)\,ds$ for $t\ge 0$. The integral on the right side of this equality is continuous, hence $h$ is continuous; we now deduce (Fundamental Theorem of Calculus) that the integral on the right side is in fact continuously differentiable. Differentiating we find that $h'(t)=\lambda h(t)$, and clearly $h(0)=0$. The unique solution of this ODE is $h(t)=0$ for all $t$. Thus your integral equation has a unique solution, given by the earlier formula.