It is shown here, without using stochastic calculus, that if $W_t$ is a standard Brownian motion, then $$ f(W_t)-\frac{1}{2}\int_0^t f''(W_s)ds $$ is a martingale, where $f\in C^2$ and compactly supported. According to Problem 4.4 in Chapter 5 of Karatzas and Shreve's Brownian Motion and Stochastic Calculus, the converse is also true. Namely, if $W$ is a continuous adapted process with $W_0=0$ such that $$ f(W_t)-\frac{1}{2}\int_0^t f''(W_s)ds\tag{1} $$ is a martingale whenever $f\in C^2$ and compactly supported, then $W$ is a standard Brownian motion. This is essentially part of the proof of Lévy's characterization of Brownian motion.
A variation of Lévy's characterization of Brownian motion
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Step 1:
The assumption holds for all $f \in C_b^2$, i.e. functions which are twice differentiable and have bounded continuous derivatives.
To this end, pick a function $\chi \in C^2_b$ such that $0 \leq \chi \leq 1$, $\chi(x) = 1$ for $x \in (-1,1)$ and $\chi(x)=0$ for all $|x|>2$. Then the function
$$f_n(x) := f(x) \cdot \chi \left( \frac{x}{n} \right), \qquad x \in \mathbb{R}, \tag{1}$$
is twice differentiable and has compact support. By assumption,
$$\mathbb{E} \left( f_n(W_t) - \frac{1}{2} \int_0^t f_n''(W_r) \, dr \mid \mathcal{F}_s \right) = f_n(W_s) - \frac{1}{2} \int_0^s f_n''(W_r) \, dr$$
for all $s \leq t$. Definition $(1)$ implies that $$\|f_n\|_{\infty}+ \|f_n'\|_{\infty} + \|f_n''\|_{\infty} \leq C$$ for some constant $C>0$ (which does not depend on $n$). Therefore, it follows from the continuity of $f,f''$ and the (conditional) dominated convergence theorem that
$$\mathbb{E} \left( f(W_t) - \frac{1}{2} \int_0^t f''(W_r) \, dr \mid \mathcal{F}_s \right) = f(W_s) - \frac{1}{2} \int_0^s f''(W_r) \, dr.$$
Step 2:
$(W_t)_{t \geq 0}$ is a Brownian motion.
The claim is proved in this answer (note that, by the first step, the assumptions in the linked question are satisfied). Don't hesitate to ask if you don't get along with it (notation is a bit different there).
A martingale is characterized by its characteristic function.
whose solution is $$ g(u, t) = \exp\left(-\frac 12 u^2 t\right) $$
From this, you get that $W$ is a brownian motion.