I have a $2n \times 2n$ real anti-triangular (skew-triangular?) block matrix of the form
$$ M = \begin{bmatrix} A & B \\ I_n & O_n \end{bmatrix} $$
where $I_n$ is the $n \times n$ identity matrix and $O_n$ is the $n \times n$ zero matrix. Note that the blocks $A$ and $B$ are also $n \times n$. Do the eigenvalues of $M$ have any specific relationships with the submatrices of $A$, $B$ (or their eigenvalues)?
Any theory or discussion would be helpful.
Nope as your matrix doesn't need to have a single eigenvalue in general, look at the trivial case where $n=1$ and your blockmatrix is
$$\begin{pmatrix}0 & -1 \\ 1 & 0 \\ \end{pmatrix}$$
As the characteristic polynomial is $x^2+1$ it doesn't have any eigenvalues over $\mathbb{R}$ while $A$ hast the eigenvalue $0$ and $B$ has the eigenvalue $-1$.