I want a proof (preferably combinatorial) of the identity
$$ \sum_{k=1}^{\lfloor n/2\rfloor} (-1)^{k-1}k\binom{n-k}{k}2^{n-2k} = \binom{n+1}{3}. $$
I suspect that there is an inclusion-exclusion proof for a family of tilings of certain board with squares and dominoes. I believe so, because the summation index $k$ is at most $\lfloor \frac{n}{2}\rfloor $ and we know that the number of tilings of an $1\times n$ board with $k$ dominoes and $n-k$ squares is $\binom{n-k}{k}$. Perhaps we can look at squares colored in 2 possible colors.
However, I cannot see how can we define this family of tilings since each term in the summation depends on $k$ and the definition of each set should be, of course, independent of the summation index $k$. We might also use that $\binom{n+1}{3} = \binom{n}{3} + \binom{n}{2}$.
Identity 173 in Proofs That Really Count: The Art of Combinatorial Proof is similar: $$\sum_{k\ge 0} (-1)^k \binom{n-k}{k}2^{n-2k} = n+1.$$ The book gives two combinatorial proofs. The first one uses two-colorings of square-domino tilings (like you suggested), and the second one uses two-colorings of square tilings, as in Combinatorial proof $\sum_i^{\lfloor{n/2}\rfloor} (-1)^i {n-i\choose i} 2^{n-2i} = n+1$
Here's a snake-oil proof of your identity: \begin{align} &\quad \sum_{n \ge 0} \left(\sum_{k=1}^{\lfloor n/2 \rfloor} (-1)^{k-1} k\binom{n-k}{k}2^{n-2k} \right) z^n \\ &= \sum_{n \ge 0} \sum_{k\ge 0}(-1)^{k-1} k\binom{n-k}{k}2^{n-2k} z^n \\ &= \sum_{k\ge 0}(-1)^{k-1} k \sum_{n \ge 2k} \binom{n-k}{k}2^{n-2k} z^n \\ &= \sum_{k\ge 0}(-1)^{k-1} k \sum_{n \ge 0} \binom{n+k}{k}2^n z^{n+2k} \\ &= \sum_{k\ge 0}(-1)^{k-1} k z^{2k} \sum_{n \ge 0} \binom{n+k}{k} (2z)^n \\ &= \sum_{k\ge 0}(-1)^{k-1} k z^{2k} \frac{1}{(1-2z)^{k+1}} \\ &= -\frac{1}{1-2z}\sum_{k\ge 0} k\left(\frac{-z^2}{1-2z}\right)^k \\ &= -\frac{1}{1-2z}\cdot\frac{-z^2/(1-2z)}{(1+z^2/(1-2z))^2} \\ &= \frac{z^2}{(1-z)^4} \\ &= z^2 \sum_{n \ge 0} \binom{n+3}{3} z^n \\ &= \sum_{n \ge 0} \binom{n+1}{3} z^n \end{align}