I'm teaching myself math. I was working on $y = -x^2 + 8x$ and I used the quadratic equation to determine the x-coordinates. I ended up with $(0,0)$ and $(-8,0)$. The vertex is $(4,16)$ and the y-intercept is $(0,0)$. The thing is, x coordinate of root should be a positive number$=+8,$ otherwise I cannot have a parabola.
Where is my error?

You have that $-x^2 + 8x = -x(x-8)$ so the intersections of the parabola with the $x$-axis are $(0,0)$ and $(8,0)$ as you remarked. You probably made a sign mistake somewhere along your computations.