For which structure $s$ (by which I mean relations or functions on $\mathbb R$) is the structure $(\mathbb R, <, s)$ isomorphic to $(\mathbb R, >, s)$ (for example by $x\mapsto - x$)?
More specifically, is the $3$-ary relation $|\cdot - \cdot| < \cdot$ such an $s$?
One simple way to establish negative results is to look at what's definable in the reduct $(\mathbb{R};s)$. For example, we have:
Proof. For the initial claim, consider the sentence "$\forall x\exists y(x\triangleleft y\wedge y\in X)$." This is true in $(\mathbb{R};<,X)$ but not in $(\mathbb{R};>,X)$. (Here "$\triangleleft$" is the ordering in the respective structure - that is, the symbol whose interpretation is $<$ or $>$ respectively.) The "consequently" then follows immediately by replacing in the sentence above the symbol $X$ with the appropriate definition of $X$ in $(\mathbb{R};s)$.
The claim above resolves your more particular question: taking $s$ to be the ternary relation $$s(a,b,c)\iff\vert a-b\vert<c,$$ note that the set of positive reals is definable in $(\mathbb{R};s$) since $c$ is positive iff we have $s(a,a,c)$ for all/some $a$.
It's worth noting that in the claim above and its application we only used the fact that first-order logic is isomorphism-invariant. In general, we can use arbitrary isomorphism-invariant logics and their equivalence notions to prove non-isomorphism results. First-order logic is merely the most important of these.