How to measure the understanding level of a new concept that was taught to a group of students

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Let us say I am an online teacher and I have to introduce a new topic in the class today. Let us say the topic is "Solving a Quadratic Equation". There is an online community of students from different backgrounds in the class and there are approx 100 students in the class.

At the end of the class I take a short quiz to see how much the students have understood this new concept.

If I want to measure the 'understanding level' of each student on the basis of the quiz's result and quantify it then how would i do that?

How can I use any concepts like means, standard deviation, poisson's or normal distribution?