We can show that the graph of the quadratic equation $y=ax^2+bx+c$ has the line of symmetry $x=-b/2a$. But how can we show that this is unique? (That is, why is no other line $dx+ey+f=0$ a line of symmetry?)
(I've been trying to show that no other line of symmetry can work but have simply been drowning in a sea of algebra. I imagine there must be some cleverer way to do it.)
Symmetry around $x=s$ is expressed by
$$ax^2+bx+c=a(2s-x)^2+b(2s-x)+c$$ for all $x$.
By identification of the powers of $x$, we get the compatibility condition
$$b+2as=0$$ or $$s=-\frac b{2a}.$$
Consider an arbitrary parabola and an arbitrary line. By rotation we can bring the line on the axis $y$. Then the equation of the rotated parabola
$$ax^2+2bxy+cy^2+2dx+2ey+f=0$$ must be invariant to a change of the sign of $x$ and it must reduce to
$$ax^2+cy^2+2ey+f=0.$$
For this equation to represent a parabola, we must have $c=0$. Then,
$$ax^2+2ey+f=0$$ is a parabola for which the axis of symmetry is $y$. Hence any line which is an axis of symmetry is the standard axis.
Note that in the case $ac\ne0$ (centered conic), by translation we can further reduce to
$$ax^2+cy^2+f=0$$ and the line is one of the known axis of symmetry. Hence there are exactly two of them.