Consider two independent real random variables $Z_1, Z_2$ and a real function $h:\mathbb{R}^2\to\mathbb{R}$ such that $\lim_{u\to\infty}h(u, z_2) = \infty$ for any $z_2\in\mathbb{R}$. Does it hold that for any $c\in\mathbb{R}$ is $$ \lim_{u\to\infty} P(h(Z_1, Z_2)\leq c\mid Z_1>u)=0? $$
I feel like an approach using nested conditional expectation should be helpful, but I can not formalize it properly. Also, should $h$ be continuous for this to be valid?
Yes (no need for continuity of $h$). I will assume that $\mathbb P(Z_1>u)>0$ for all $u\in\mathbb R$, so that the conditional probability is well defined.
Let $c\in\mathbb R$. Denoting by $\mu$ the distribution of $Z_2$, we have that $$ \begin{align*} \mathbb P(h(Z_1,Z_2)\le c\vert Z_1>u)&=\frac{\mathbb P(h(Z_1,Z_2)\le c,Z_1>u)}{\mathbb P(Z_1>u)}\\ &=\int_{\mathbb R}\frac{\mathbb P(h(Z_1,z_2)\le c,Z_1>u)}{\mathbb P(Z_1>u)}\,\mu(dz_2). \end{align*} $$
Let $z_2\in\mathbb R$. As $h(u,z_2)$ goes to $+\infty$ with $u$, the integrand is $0$ for $u$ large enough. We deduce that the integrand converges pointwise to $0$ as $u\to+\infty$. As it is bounded by $1$, the dominated convergence theorem yields that the integral converges to $0$ as $u\to+\infty$.